Quantitative Assay of Interferon by the Immunofluorescent Cell-counting Technique
نویسندگان
چکیده
منابع مشابه
Quantitative assay of interferon by the immunofluorescent cell-counting technique.
A rapid, quantitative assay of interferon was developed, based on the reduction of the number of virus-infected cells by dilutions of interferon. Infected cells were enumerated by fluorescent cell-counting 24 hours after challenge of interferontreated McCoy cover-slip cell monolayers. A quantitative linear relationship between the reduction of fluorescent cells and dilutions of interferon was d...
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A quantitative assay for lymphocytic choriomeningitis virus was developed and standardized. The assay is based on direct immunofluorescent staining of infected L-929 cell monolayers and enumeration of cells containing fluorescent viral antigens. Maximal adsorption of virus to cells occurred within 1 h. Observations on the sequential development of viral antigens within cells showed that specifi...
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A quantitative assay for infective variola virus particles was developed which is based on the enumeration of cells containing fluorescent viral antigen after infection of McCoy cell monolayers. The direct fluorescent-antibody technique was employed to stain cells. The efficiency of virus adsorption was markedly enhanced by centrifugation of virus inoculum onto McCoy cell monolayers at 500 x g ...
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The studies described here indicate that the fluorescent cellcounting technique provides a rapid, precise, reproducible, and sensitive procedure for the assay of infective particles of psittacosis virus. The efficiency of virus adsorption onto coveralip cultures of McCoy cells was markedly increased by the use of centrifugal force; assay values were 1.0 log higher than those obtained after stat...
متن کاملComparison of the immunofluorescent-cell counting and plaque methods for the assay of vaccinia virus.
The immunofluorescent-cell counting method was compared with the agarand liquid-overlay plaque techniques for the assay of vaccinia virus. In addition to being as quantitative, reproducible, and simple to use as the two plaque techniques, the immunofluorescent assay method was found to be more sensitive, rapid, and specific.
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1969
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-4-3-441